Espécies de Botryosphaeriaceae associadas à podridão em seriguela.

Abstract

The peduncular rot is one of the main diseases that compromise the commercialization of seriguela. This disease is commonly associated with Lasiodiplodia theobromae, however, studies report other species of Botryosphaeriaceae associated with this symptom in other fruits. The objective of this research was to identify Botryosphaeriaceae species associated with fruit rot in seriguela in the states of Pernambuco and Ceará. Seriguela fruits were collected in productive backyards and small rural properties in both states. The fruits were superficially disinfected and placed in a humid chamber under laboratory conditions until the appearance of rot symptoms. The fruits with symptoms were submitted to the isolation process. Then, the pathogenicity test of the fungal isolates obtained from ripe seriguela fruits without disease symptoms was performed. The pathogenic isolates were subjected to extraction and amplification of genomic DNA from the ITS region and from the EF1-α and TUB genes. The sequences of the 79 representative isolates were compared with reference sequences from fungal species of the Botryosphaeriaceae family. The isolates were grouped in clades with species of different genera of Botryosphaeriaceae. Most of the isolates belong to the genus Lasiodiplodia and L. iraniensis is the Botryosphaeriaceae species most frequently associated with peduncular rot of seriguela fruits in the states of Permanbuco and Ceará. The species Diplodia eriobotryicola was also found associated with this rot, being the first report of this species in Brazil.